O-Specific Polysaccharide-Specific Memory B Cell Responses in Young Children, Older Children, and Adults Infected with Vibrio cholerae O1 Ogawa in Bangladesh
Author
Aktar, Amena
Rahman, M. Arifur
Afrin, Sadia
Faruk, M. Omar
Uddin, Taher
Akter, Aklima
Sami, Israk Nur
Yasmin, Tahirah
Chowdhury, Fahima
Khan, Ashraful
Leung, Daniel
LaRocque, Regina
Charles, Richelle
Bhuiyan, Taufiqur Rahman
Mandlik, Anjali
Kelly, Meagan
Kováč, Pavol
Xu, Peng
Calderwood, Stephen
Harris, Jason
Qadri, Firdausi
Ryan, Edward
Published Version
https://doi.org/10.1128/CVI.00647-15Metadata
Show full item recordCitation
Aktar, Amena, M. Arifur Rahman, Sadia Afrin, M. Omar Faruk, Taher Uddin, Aklima Akter, M. Israk Nur Sami, et al. 2016. “O-Specific Polysaccharide-Specific Memory B Cell Responses in Young Children, Older Children, and Adults Infected with Vibrio Cholerae O1 Ogawa in Bangladesh.” Edited by R. S. Abraham. Clinical and Vaccine Immunology 23 (5): 427–35. https://doi.org/10.1128/CVI.00647-15.Abstract
Cholera caused by Vibrio cholerae O1 confers at least 3 to 10 years of protection against subsequent disease regardless of age, despite a relatively rapid fall in antibody levels in peripheral blood, suggesting that memory B cell responses may play an important role in protection. The V. cholerae O1-specific polysaccharide (OSP) component of lipopolysaccharide (LPS) is responsible for serogroup specificity, and it is unclear if young children are capable of developing memory B cell responses against OSP, a T cell-independent antigen, following cholera. To address this, we assessed OSP-specific memory B cell responses in young children (2 to 5 years, n = 11), older children (6 to 17 years, n = 21), and adults (18 to 55 years, n = 28) with cholera caused by V. cholerae O1 in Dhaka, Bangladesh. We also assessed memory B cell responses against LPS and vibriocidal responses, and plasma antibody responses against OSP, LPS, and cholera toxin B subunit (CtxB; a T cell-dependent antigen) on days 2 and 7, as well as days 30, 90, and 180 after convalescence. In all age cohorts, vibriocidal responses and plasma OSP, LPS, and CtxB-specific responses peaked on day 7 and fell toward baseline over the follow-up period. In comparison, we were able to detect OSP memory B cell responses in all age cohorts of patients with detectable responses over baseline for 90 to 180 days. Our results suggest that OSP-specific memory B cell responses can occur following cholera, even in the youngest children, and may explain in part the age-independent induction of long-term immunity following naturally acquired disease.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41542655
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)