Identification of a Robust Antibody Modulator of CD33 in In Vitro Models
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Aiello, Arica Lynn
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Aiello, Arica Lynn. 2023. Identification of a Robust Antibody Modulator of CD33 in In Vitro Models. Master's thesis, Harvard University Division of Continuing Education.Abstract
CD33 is a sialic acid binding immunoglobulin-type lectin found on myeloid cells throughout the body, including microglia, the brain’s resident immune cells. CD33 has been implicated genetically in late-onset Alzheimer’s disease (LOAD) as both a potential risk factor and potential protective factor. This is based on the alternative splicing of CD33, which produces two isoforms of the protein: a full-length isoform, and a shorter isoform lacking the extracellular sialic acid binding domain. Because of its implication in disease, CD33 may be a potential therapeutic target for AD. A previous study by Wißfeld, et al. (2021) has designed a reporter cell assay capable of monitoring CD33 activation. Using this model, we were able to identify a robust antibody modulator of CD33, rabbit monoclonal E6V7H. Endogenous models expressing CD33, THP-1 and TF- 1, were also tested using E6V7H for CD33 activation. We found that treating these cells with E6V7H altered the expression levels of downstream phospho-SHP-1, but more work needs to be done on the downstream signaling of CD33 to create an endogenous readout of CD33 activation.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37377207
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