Highly Multiplexed Subcellular RNA Sequencing in Situ
Author
Lee, Je Hyuk
Kalhor, Reza
Yang, Joyce
Terry, Richard
Jeanty, Sauveur
Li, Chao
Turczyk, Brian
Marblestone, Adam
Inverso, Samuel
Bernard, Amy
Mali, Prashant
Rios, Xavier
Published Version
https://doi.org/10.1126/science.1250212Metadata
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Lee, Je Hyuk, Evan Daugharthy, Jonathan Scheiman, Reza Kalhor, Thomas Ferrante, Joyce Yang, Richard Terry et al. "Highly Multiplexed Subcellular RNA Sequencing in Situ." Science 343, no. 6177 (2014): 1360-1363. DOI: 10.1126/science.1250212Abstract
Understanding the spatial organization of gene expression with single nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here we describe fluorescent in situ RNA sequencing (FISSEQ), in which stably cross-linked cDNA amplicons are sequenced within a biological sample. Using 30-base reads from 8,742 genes in situ, we examined RNA expression and localization in human primary fibroblasts using a simulated wound healing assay. FISSEQ is compatible with tissue sections and whole mount embryos, and reduces the limitations of optical resolution and noisy signals on single molecule detection. Our platform enables massively parallel detection of genetic elements, including gene transcripts and molecular barcodes, and can be used to investigate cellular phenotype, gene regulation, and environment in situ.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140943/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369313
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