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dc.contributor.authorLeiman, Sara A
dc.contributor.authorMay, J. M.
dc.contributor.authorLebar, M. D.
dc.contributor.authorKahne, Daniel
dc.contributor.authorKolter, R.
dc.contributor.authorLosick, Richard M.
dc.date.accessioned2017-11-02T19:05:02Z
dc.date.issued2013
dc.identifierQuick submit: 2017-05-11T11:58:00-0400
dc.identifier.citationLeiman, S. A., J. M. May, M. D. Lebar, D. Kahne, R. Kolter, and R. Losick. 2013. “D-Amino Acids Indirectly Inhibit Biofilm Formation in Bacillus Subtilis by Interfering with Protein Synthesis.” Journal of Bacteriology 195 (23) (October 4): 5391–5395. doi:10.1128/jb.00975-13.en_US
dc.identifier.issn0021-9193en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34323222
dc.description.abstractThe soil bacterium Bacillus subtilis forms biofilms on surfaces and at air-liquid interfaces. It was previously reported that these biofilms disassemble late in their life cycle and that conditioned medium from late-stage biofilms inhibits biofilm formation. Such medium contained a mixture of d-leucine, d-methionine, d-tryptophan, and d-tyrosine and was reported to inhibit biofilm formation via the incorporation of these d-amino acids into the cell wall. Here, we show that l-amino acids were able to specifically reverse the inhibitory effects of their cognate d-amino acids. We also show that d-amino acids inhibited growth and the expression of biofilm matrix genes at concentrations that inhibit biofilm formation. Finally, we report that the strain routinely used to study biofilm formation has a mutation in the gene (dtd) encoding d-tyrosyl-tRNA deacylase, an enzyme that prevents the misincorporation of d-amino acids into protein in B. subtilis. When we repaired the dtd gene, B. subtilis became resistant to the biofilm-inhibitory effects of d-amino acids without losing the ability to incorporate at least one noncanonical d-amino acid, d-tryptophan, into the peptidoglycan peptide side chain. We conclude that the susceptibility of B. subtilis to the biofilm-inhibitory effects of d-amino acids is largely, if not entirely, due to their toxic effects on protein synthesis.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.isversionofdoi:10.1128/JB.00975-13en_US
dash.licenseLAA
dc.titleD-Amino Acids Indirectly Inhibit Biofilm Formation in Bacillus subtilis by Interfering with Protein Synthesisen_US
dc.typeJournal Articleen_US
dc.date.updated2017-05-11T15:57:29Z
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Bacteriologyen_US
dash.depositing.authorKahne, Daniel
dc.date.available2013
dc.date.available2017-11-02T19:05:02Z
dc.identifier.doi10.1128/JB.00975-13*
dash.identifier.orcid0000-0002-3937-5964en_US
dash.contributor.affiliatedLeiman, Sara
dash.contributor.affiliatedLosick, Richard
dash.contributor.affiliatedKahne, Daniel
dc.identifier.orcid0000-0002-3937-5964


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