dc.contributor.author | Leiman, Sara A | |
dc.contributor.author | May, J. M. | |
dc.contributor.author | Lebar, M. D. | |
dc.contributor.author | Kahne, Daniel | |
dc.contributor.author | Kolter, R. | |
dc.contributor.author | Losick, Richard M. | |
dc.date.accessioned | 2017-11-02T19:05:02Z | |
dc.date.issued | 2013 | |
dc.identifier | Quick submit: 2017-05-11T11:58:00-0400 | |
dc.identifier.citation | Leiman, S. A., J. M. May, M. D. Lebar, D. Kahne, R. Kolter, and R. Losick. 2013. “D-Amino Acids Indirectly Inhibit Biofilm Formation in Bacillus Subtilis by Interfering with Protein Synthesis.” Journal of Bacteriology 195 (23) (October 4): 5391–5395. doi:10.1128/jb.00975-13. | en_US |
dc.identifier.issn | 0021-9193 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:34323222 | |
dc.description.abstract | The soil bacterium Bacillus subtilis forms biofilms on surfaces and at air-liquid interfaces. It was previously reported that these biofilms disassemble late in their life cycle and that conditioned medium from late-stage biofilms inhibits biofilm formation. Such medium contained a mixture of d-leucine, d-methionine, d-tryptophan, and d-tyrosine and was reported to inhibit biofilm formation via the incorporation of these d-amino acids into the cell wall. Here, we show that l-amino acids were able to specifically reverse the inhibitory effects of their cognate d-amino acids. We also show that d-amino acids inhibited growth and the expression of biofilm matrix genes at concentrations that inhibit biofilm formation. Finally, we report that the strain routinely used to study biofilm formation has a mutation in the gene (dtd) encoding d-tyrosyl-tRNA deacylase, an enzyme that prevents the misincorporation of d-amino acids into protein in B. subtilis. When we repaired the dtd gene, B. subtilis became resistant to the biofilm-inhibitory effects of d-amino acids without losing the ability to incorporate at least one noncanonical d-amino acid, d-tryptophan, into the peptidoglycan peptide side chain. We conclude that the susceptibility of B. subtilis to the biofilm-inhibitory effects of d-amino acids is largely, if not entirely, due to their toxic effects on protein synthesis. | en_US |
dc.description.sponsorship | Chemistry and Chemical Biology | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Society for Microbiology | en_US |
dc.relation.isversionof | doi:10.1128/JB.00975-13 | en_US |
dash.license | LAA | |
dc.title | D-Amino Acids Indirectly Inhibit Biofilm Formation in Bacillus subtilis by Interfering with Protein Synthesis | en_US |
dc.type | Journal Article | en_US |
dc.date.updated | 2017-05-11T15:57:29Z | |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Journal of Bacteriology | en_US |
dash.depositing.author | Kahne, Daniel | |
dc.date.available | 2013 | |
dc.date.available | 2017-11-02T19:05:02Z | |
dc.identifier.doi | 10.1128/JB.00975-13 | * |
dash.identifier.orcid | 0000-0002-3937-5964 | en_US |
dash.contributor.affiliated | Leiman, Sara | |
dash.contributor.affiliated | Losick, Richard | |
dash.contributor.affiliated | Kahne, Daniel | |
dc.identifier.orcid | 0000-0002-3937-5964 | |