Loss of RNA expression and allele-specific expression associated with congenital heart disease
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Patel, Neil
Ware, James S.
Zaidi, Samir
Ma, Wenji
Patel, Nihir
Lifton, Richard P.
Chung, Wendy K.
Kim, Richard
Shen, Yufeng
Brueckner, Martina
Goldmuntz, Elizabeth
Sharp, Andrew J.
Gelb, Bruce D.
Seidman, J. G.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/ncomms12824Metadata
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McKean, D. M., J. Homsy, H. Wakimoto, N. Patel, J. Gorham, S. R. DePalma, J. S. Ware, et al. 2016. “Loss of RNA expression and allele-specific expression associated with congenital heart disease.” Nature Communications 7 (1): 12824. doi:10.1038/ncomms12824. http://dx.doi.org/10.1038/ncomms12824.Abstract
Congenital heart disease (CHD), a prevalent birth defect occurring in 1% of newborns, likely results from aberrant expression of cardiac developmental genes. Mutations in a variety of cardiac transcription factors, developmental signalling molecules and molecules that modify chromatin cause at least 20% of disease, but most CHD remains unexplained. We employ RNAseq analyses to assess allele-specific expression (ASE) and biallelic loss-of-expression (LOE) in 172 tissue samples from 144 surgically repaired CHD subjects. Here we show that only 5% of known imprinted genes with paternal allele silencing are monoallelic versus 56% with paternal allele expression—this cardiac-specific phenomenon seems unrelated to CHD. Further, compared with control subjects, CHD subjects have a significant burden of both LOE genes and ASE events associated with altered gene expression. These studies identify FGFBP2, LBH, RBFOX2, SGSM1 and ZBTB16 as candidate CHD genes because of significantly altered transcriptional expression.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052634/pdf/Terms of Use
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