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Bioproduction of D-Tagatose in Escherichia coli by Harnessing the Reverse Leloir Pathway
(2023-01-11)
D-Tagatose is a natural, rare monosaccharide containing 38% of the calories of sucrose. As an alternative sweetener, it offers numerous benefits including a low glycemic index, prebiotic properties, and the ability to lower ...
Addressing the Needs of Research and Clinical Applications for Cell-Free DNA, Exome Selection, and Targeted Panel Selection in a High-Throughput Laboratory Environment
(2021-10-20)
The thesis will address the development of a new targeted enrichment method using a hybrid selection-based approach. While targeted enrichment approaches have been used routinely by many research and clinical labs, the ...
Identifying Circulating MiRNAs as Potential Biomarkers for Kidney Disease by qRT-PCR
MicroRNAs (miRNAs) are short endogenous non-coding RNA molecules that play critical roles in gene regulation, cell differentiation, proliferation, apoptosis, and metabolism. MiRNA levels in blood and other body fluids are ...
An Investigation into the Genetic and Bacterial Factors That Cause Irritable Bowel Syndrome and a Solution in the Form of a Targeted Next Generation Sequencing Compatible Gene Panel for the Disease.
(2023-05-01)
Irritable Bowel Syndrome (IBS) is a prevalent disease without complete
mechanistic understanding. Medical doctors and scientists alike do not know the cause
for the disease and therefore have trouble accurately diagnosing ...
Recombinant Protein Production using a Mammalian Unconventional Secretory Pathway
(2023-10-19)
Recombinant proteins are widely used throughout biomedical research, and the expansive and diversified usage of such reagents requires continued development of novel production processes. Unconventional protein secretion ...