Browsing by Author "Kahne, Daniel"
Now showing items 21-35 of 35
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Oxidative Assembly of the Outer Membrane Lipopolysaccharide Translocon LptD/E and Progress towards Its X-Ray Crystal Structure
Garner, Ronald Aaron (2014-10-21)Lipopolysaccharide (LPS) is the glycolipid that comprises the outer leaflet of the Gram-negative outer membrane (OM). Because it is essential in nearly all Gram-negative species, and because it is responsible for making ... -
Reconstitution of bacterial lipopolysaccharide transport from purified components
Sherman, David Joseph (2015-05-18)The surface of Gram-negative bacteria is largely composed of lipopolysaccharide (LPS), a complex glycolipid that contains multiple fatty acyl chains and up to hundreds of sugars. LPS is transported from its site of synthesis ... -
Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis
Lebar, Matthew D.; May, Janine M.; Meeske, Alexander J.; Leiman, Sara A.; Lupoli, Tania J.; Tsukamoto, Hirokazu; Losick, Richard; Rudner, David Z.; Walker, Suzanne; Kahne, Daniel (American Chemical Society, 2014)The peptidoglycan precursor, Lipid II, produced in the model Gram-positive bacterium Bacillus subtilis differs from Lipid II found in Gram-negative bacteria such as Escherichia coli by a single amidation on the peptide ... -
Reconstitution of the E. Coli Membrane \(\beta\)-Barrel Assembly Machine from Purified Components
Hagan, Christine Lepicier (2012-09-14)\(\beta\)-barrel membrane proteins perform important functions in the outer membranes of Gram-negative bacteria and in the mitochondria and chloroplasts of eukaryotes. Cellular machines that have been conserved from bacteria ... -
Reconstitution of the Final Step of Peptidoglycan Assembly in Staphylococcus aureus
Qiao, Yuan (2016-05-10)Bacterial peptidoglycan (PG) is an exoskeleton structure that maintains cell shape and protects cells from lysis. Peptidoglycan is essential in bacteria but is not found in mammalian cells. Therefore, it is the target for ... -
Single-molecule dynamics show a transient lipopolysaccharide transport bridge
Törk, Lisa; Moffatt, Caitlin B.; Bernhardt, Thomas G.; Garner, Ethan C.; Kahne, Daniel (Springer Science and Business Media LLC, 2023-11-08) -
Spatial Organizing Principles of Cytokinesis Signaling
Nguyen, Phuong Anh (2015-09-23)Cytokinesis is the final step of cell division, in which the cytoplasm of one cell is physically divided into two. Animal cells integrate multiple positional cues from the mitotic spindle to decide where to position their ... -
Structural and Functional Studies Into Nramp Divalent Metal Transporter Mechanisms
Bane, Lukas (2015-07-10)The Nramp (natural resistance associated macrophage protein) family of transition metal ion transporters is an important class of secondary active transporters involved in metal homeostasis used by all branches of life. ... -
Structural Basis of Unidirectional Export of Lipopolysaccharide to the Cell Surface
Kahne, Daniel; Owens, Tristan; Taylor, Rebecca; Pahil, Karanbir; Bertani, Blake; Ruiz, Natividad; Kruse, Andrew (Springer Science and Business Media LLC, 2019-03)Gram-negative bacteria are surrounded by an inner cytoplasmic membrane and by an outer membrane, which serves as a protective barrier to limit entry of many antibiotics. The distinctive properties of the outer membrane are ... -
Structural Characterization of BCL-2 Family Protein Interactions Using Photoreactive Stapled Peptides and Mass Spectrometry
Braun, Craig Ronald (2013-02-22)Recent improvements in mass spectrometry instrumentation have stimulated the fusion of this technology with protein crosslinking to advance the structural proteomics field. However, analysis of complex datasets from ... -
Structure and Reconstitution of a Hydrolase Complex That May Release Peptidoglycan From the Membrane After Polymerization
Schaefer, Kaitlin; Owens, Tristan W.; Page, Julia; Santiago, Marina; Kahne, Daniel; Walker, Suzanne (Springer Science and Business Media LLC, 2021-01)Bacteria are surrounded by a peptidoglycan cell wall that is essential for their survival1. During cell wall assembly, a lipid-linked disaccharide-peptide precursor called Lipid II is polymerized and crosslinked to produce ... -
Structure of a Nascent Membrane Protein as It Folds on the BAM Complex
Tomasek, David; Rawson, Shaun; Lee, James; Wzorek, Joseph; Harrison, Stephen; Li, Zongli; Kahne, Daniel (Springer Science and Business Media LLC, 2020-06-11) -
Studies towards a Solution Structure of the Peptidoglycan Glycosyltransferases
Wu, Yihui (2012-09-13)Peptidoglycan glycosyltransferases (PGTs) are highly conserved bacterial enzymes that catalyze the polymerization of the lipidic disaccharide, Lipid II, to form individual peptidoglycan (PG) strands which are subsequently ... -
Understanding and Preventing Disease-Associated Anaerobic Choline Metabolism by the Human Gut Microbiota
Bodea, Smaranda (2016-05-17)The consortium of microorganisms inhabiting the human gastrointestinal tract, our gut microbiota, has an extensive set of metabolic capabilities that directly influence human health. Over the past decade, DNA sequencing ... -
Using Live Cell Imaging to Probe Biogenesis of the Gram-Negative Cell Envelope
Yao, Zhizhong (2013-02-08)In Gram-negative bacteria, the three-layered cell envelope, including the cell wall, outer and inner membranes, is essential for cell survival in the changing, and often hostile environments. Conserved in all prokaryotes, ...