Browsing by Author "Mali, Prashant"
Now showing items 1-7 of 7
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Barcoding cells using cell-surface programmable DNA-binding domains
Mali, Prashant; Aach, John; Lee, Jehyuk; Levner, Daniel; Nip, Lisa; Church, George M. (2013)We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically ... -
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
Mali, Prashant; Aach, John; Stranges, P. Benjamin; Esvelt, Kevin M.; Moosburner, Mark; Kosuri, Sriram; Yang, Luhan; Church, George M. (2013)Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.1–7. Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering ... -
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
DiCarlo, James; Norville, Julie; Mali, Prashant; Rios Villanueva, Xavier; Aach, John Dennis; Church, George McDonald (Oxford University Press, 2013)Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic ... -
Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers
Briggs, Adrian; Rios, Xavier; Chari, Rajagopal; Yang, Luhan; Zhang, Feng; Mali, Prashant; Church, George McDonald (Oxford University Press, 2012)DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers ... -
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells
Byrne, Susan M.; Ortiz, Luis; Mali, Prashant; Aach, John; Church, George M. (Oxford University Press, 2015)Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient ‘knock-in’ targeted ... -
Optimization of scarless human stem cell genome editing
Yang, Luhan; Guell, Marc; Byrne, Susan; Yang, Joyce L.; De Los Angeles, Alejandro; Mali, Prashant; Aach, John; Kim-Kiselak, Caroline; Briggs, Adrian W; Rios, Xavier; Huang, Po-Yi; Daley, George; Church, George (Oxford University Press, 2013)Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific ... -
Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing
Esvelt, Kevin M.; Mali, Prashant; Braff, Jonathan L.; Moosburner, Mark; Yaung, Stephanie J.; Church, George M. (2013)The Cas9 protein from the Streptococcus pyogenes CRISPR-Cas immune system has been adapted for both RNA-guided genome editing and gene regulation in a variety of organisms, but can mediate only a single activity at a time ...