Browsing by Author "Guan, Yinghua"

Now showing items 1-5 of 5

    • High-Affinity Accumulation of a Maytansinoid in Cells via Weak Tubulin Interaction 

      Goldmacher, Victor S.; Audette, Charlene A.; Guan, Yinghua; Sidhom, Eriene-Heidi; Shah, Jagesh V.; Whiteman, Kathleen R.; Kovtun, Yelena V. (Public Library of Science, 2015)
      The microtubule-targeting maytansinoids accumulate in cells and induce mitotic arrest at 250- to 1000-fold lower concentrations than those required for their association with tubulin or microtubules. To identify the ...

    • Impact of Histone H4 Lysine 20 Methylation on 53BP1 Responses to Chromosomal Double Strand Breaks 

      Hartlerode, Andrea J.; Guan, Yinghua; Rajendran, Anbazhagan; Ura, Kiyoe; Schotta, Gunnar; Xie, Anyong; Shah, Jagesh V.; Scully, Ralph (Public Library of Science, 2012)
      Recruitment of 53BP1 to chromatin flanking double strand breaks (DSBs) requires γH2AX/MDC1/RNF8-dependent ubiquitination of chromatin and interaction of 53BP1 with histone H4 methylated on lysine 20 (H4K20me). Several ...

    • Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein 

      Guan, Yinghua; Meurer, Matthias; Raghavan, Sarada; Rebane, Aleksander; Lindquist, Jake R.; Santos, Sofia; Kats, Ilia; Davidson, Michael W.; Mazitschek, Ralph; Hughes, Thomas E.; Drobizhev, Mikhail; Knop, Michael; Shah, Jagesh V. (The American Society for Cell Biology, 2015)
      We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal ...

    • LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair 

      Liu, Xue-Song; Chandramouly, Gurushankar; Rass, Emilie; Guan, Yinghua; Wang, Guocan; Hobbs, Robin M.; Rajendran, Anbazhagan; Xie, Anyong; Shah, Jagesh V.; Davis, Anthony J.; Scully, Ralph; Lunardi, Andrea; Pandolfi, Pier Paolo (Nature Pub. Group, 2015)
      Leukemia/lymphoma-related factor (LRF) is a POZ/BTB and Krüppel (POK) transcriptional repressor characterized by context-dependent key roles in cell fate decision and tumorigenesis. Here we demonstrate an unexpected ...

    • Monoalkoxy BODIPYs—A Fluorophore Class for Bioimaging 

      Courtis, Alexandra M.; Santos, Sofia A.; Guan, Yinghua; Hendricks, J. Adam; Ghosh, Balaram; Szantai-Kis, D. Miklos; Reis, Surya A.; Shah, Jagesh V.; Mazitschek, Ralph (American Chemical Society, 2014)
      Small molecule fluorophores are indispensable tools for modern biomedical imaging techniques. In this report, we present the development of a new class of BODIPY dyes based on an alkoxy-fluoro-boron-dipyrromethene core. ...