Browsing by Author "Losick, Richard"
Now showing items 1-12 of 12
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Broadly heterogeneous activation of the master regulator for sporulation in Bacillus subtilis
Chastanet, A.; Vitkup, D.; Yuan, Guocheng; Norman, Thomas Maxwell; Liu, Jun; Losick, Richard M. (Proceedings of the National Academy of Sciences, 2010)A model system for investigating how developmental regulatory networks determine cell fate is spore formation in Bacillus subtilis. The master regulator for sporulation is Spo0A, which is activated by phosphorylation via ... -
D-Amino Acids Indirectly Inhibit Biofilm Formation in Bacillus subtilis by Interfering with Protein Synthesis
Leiman, Sara A; May, J. M.; Lebar, M. D.; Kahne, Daniel; Kolter, R.; Losick, Richard M. (American Society for Microbiology, 2013)The soil bacterium Bacillus subtilis forms biofilms on surfaces and at air-liquid interfaces. It was previously reported that these biofilms disassemble late in their life cycle and that conditioned medium from late-stage ... -
The Extracellular Matrix of Staphylococcus aureus Biofilms Comprises Cytoplasmic Proteins That Associate with the Cell Surface in Response to Decreasing pH
Foulston, Lucy; Elsholz, Alexander K. W.; DeFrancesco, Alicia S.; Losick, Richard (American Society of Microbiology, 2014)ABSTRACT Biofilm formation by Staphylococcus aureus involves the formation of an extracellular matrix, but the composition of this matrix has been uncertain. Here we report that the matrix is largely composed of cytoplasmic ... -
Galactose Metabolism Plays a Crucial Role in Biofilm Formation by Bacillus subtilis
Chai, Yunrong; Beauregard, Pascale; Vlamakis, Hera C; Losick, Richard M.; Kolter, Roberto Guillermo (American Society of Microbiology, 2012)Galactose is a common monosaccharide that can be utilized by all living organisms via the activities of three main enzymes that make up the Leloir pathway: GalK, GalT, and GalE. In Bacillus subtilis, the absence of GalE ... -
Memory and Modularity in Cell-Fate Decision Making
Norman, Thomas M.; Lord, Nathan D.; Paulsson, Johan; Losick, Richard (2014)Genetically identical cells sharing an environment can display markedly different phenotypes. It is often unclear how much of this variation derives from chance, external signals, or attempts by individual cells to exert ... -
Memory in a phenotypic switch and noise in gene networks
Norman, Thomas Maxwell (2014-02-25)Many cell types stochastically switch phenotypes under some conditions, so that genetically identical sister cells may behave quite differently in a common environment. This non-genetic variability likely arises from noise ... -
The Program of Gene Transcription for a Single Differentiating Cell Type During Sporulation in Bacillus Subtilis
Rudner, David Z; Liu, Jun; Ferguson, Caitlin; Conlon, Erin M; Losick, Richard; Wang, Stephanie T; Sato, Tsutomu; Haga, Koki; Eichenberger, Patrick; Fujita, Masaya; Jensen, Shane T (Public Library of Science, 2004)Asymmetric division during sporulation by Bacillus subtilis generates a mother cell that undergoes a 5-h program of differentiation. The program is governed by a hierarchical cascade consisting of the transcription factors: ... -
Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis
Lebar, Matthew D.; May, Janine M.; Meeske, Alexander J.; Leiman, Sara A.; Lupoli, Tania J.; Tsukamoto, Hirokazu; Losick, Richard; Rudner, David Z.; Walker, Suzanne; Kahne, Daniel (American Chemical Society, 2014)The peptidoglycan precursor, Lipid II, produced in the model Gram-positive bacterium Bacillus subtilis differs from Lipid II found in Gram-negative bacteria such as Escherichia coli by a single amidation on the peptide ... -
Respiration control of multicellularity in Bacillus subtilis by a complex of the cytochrome chain with a membrane-embedded histidine kinase
Kolodkin-Gal, I.; Elsholz, A. K. W.; Muth, C.; Girguis, Peter R.; Kolter, Roberto Guillermo; Losick, Richard M. (Cold Spring Harbor Laboratory Press, 2013)Bacillus subtilis forms organized multicellular communities known as biofilms wherein the individual cells are held together by a self-produced extracellular matrix. The environmental signals that promote matrix synthesis ... -
Sporulenes, Heptaprenyl Metabolites from Bacillus subtilis Spores
Kontnik, Renee; Bosak, Tanja; Butcher, Rebecca A.; Brocks, Jochen J.; Losick, Richard M.; Clardy, Jon C.; Pearson, Ann (American Chemical Society, 2008)Sporulene, a C35-terpenoid hydrocarbon with an unusual pentacyclic structure, is produced by Bacillus subtilis during sporulation. -
Synthesis and Activity of Biomimetic Biofilm Disruptors
Böttcher, Thomas; Kolodkin-Gal, Ilana; Kolter, Roberto Guillermo; Losick, Richard M.; Clardy, Jon C. (American Chemical Society, 2013)Biofilms are often associated with human bacterial infections, and the natural tolerance of biofilms to antibiotics challenges treatment. Compounds with antibiofilm activity could become useful adjuncts to antibiotic ... -
Transcriptional Control of Maternal-Fetal Immune Tolerance
Ferreira, Leonardo (2016-05-18)Human leukocyte antigens (HLA) are important determinants of self-nonself immune recognition. HLA-G, uniquely expressed in the placenta, is believed to be key to fetus-induced immune tolerance during pregnancy. The ...