Browsing by Author "Saulnier, Jessica"

Now showing items 1-6 of 6

    • All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins 

      Hochbaum, Daniel R.; Zhao, Yongxin; Farhi, Samouil L.; Klapoetke, Nathan; Werley, Christopher A.; Kapoor, Vikrant; Zou, Peng; Kralj, Joel M.; Maclaurin, Dougal; Smedemark-Margulies, Niklas; Saulnier, Jessica L.; Boulting, Gabriella L.; Straub, Christoph; Cho, Yong Ku; Melkonian, Michael; Wong, Gane Ka-Shu; Harrison, D. Jed; Murthy, Venkatesh N.; Sabatini, Bernardo; Boyden, Edward S.; Campbell, Robert E.; Cohen, Adam E. (2014)
      All-optical electrophysiology—spatially resolved simultaneous optical perturbation and measurement of membrane voltage—would open new vistas in neuroscience research. We evolved two archaerhodopsin-based voltage indicators, ...

    • CRISPR/Cas9-Mediated Gene Knock-Down in Post-Mitotic Neurons 

      Straub, Christoph; Granger, Adam J.; Saulnier, Jessica L.; Sabatini, Bernardo L. (Public Library of Science, 2014)
      The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual ...

    • High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons 

      Nieland, Thomas J. F.; Logan, David J.; Saulnier, Jessica; Lam, Daniel; Johnson, Caroline; Root, David E.; Carpenter, Anne E.; Sabatini, Bernardo L. (Public Library of Science, 2014)
      The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation ...

    • A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging 

      Chen, Yao; Saulnier, Jessica L.; Yellen, Gary; Sabatini, Bernardo L. (Frontiers Media S.A., 2014)
      Neuromodulators have profound effects on behavior, but the dynamics of their intracellular effectors has remained unclear. Most neuromodulators exert their function via G-protein-coupled receptors (GPCRs). One major challenge ...

    • Spatial Organization of Local Inputs to Spiny Projection Neurons in the Striatum 

      Saulnier, Jessica L. (2015-10-19)
      GABAergic interneurons are important for balanced activity of the principal projection neurons of the striatum (spiny projection neurons, SPNs) and dysfunction of striatal GABAergic interneurons can lead to movement-related ...

    • Temporal dynamics of a homeostatic pathway controlling neural network activity 

      Bateup, Helen S.; Denefrio, Cassandra L.; Johnson, Caroline A.; Saulnier, Jessica L.; Sabatini, Bernardo L. (Frontiers Media S.A., 2013)
      Neurons use a variety of mechanisms to homeostatically regulate neural network activity in order to maintain firing in a bounded range. One such process involves the bi-directional modulation of excitatory synaptic drive ...